The HPLC-HRMS combination is used for the separation and analysis of organic compounds. The substances result from a highly diverse range of synthesis techniques from a variety of research groups. They require very thorough analytics to verify the identity of new compounds and, where appropriate, the use of preparative HPLC to solve complex separation problems (e.g., diastereomer separation). Important is the determination of the molecular formula by the exact determination of the molecular masses using considerably less synthesized material than in classical quantitative elemental analysis. This HPLC-HRMS is superior to an HPLC-DAD because the detector does not rely on chromophores, which makes preparative HPLC more reliable.

What you can do with it:
Analysis of ...

• precursors of reactive intermediates and peptides
• Halogen bridge donors and catalysis products
• catalytic cycles and catalytic products
• partial syntheses and fermentations of natural products
• peptide derivatives
• chiral molecules

More details:

The HPLC-HRMS combination is a fast, flexible and highly precise analytics and is used by several working groups, who work on very different questions and perform their analyzes. These questions and analyzes are among others:

• organic molecular magnets
• cyclic peptides
• Activation of molecular hydrogen by organic molecules
• Influence of reactivity by microsolvation
• Organocatalysts based on halogen-bond donors and N-heterocyclic carbenes
• Transition-metal catalysts for organic synthesis: Identification of special starting materials, (by-) products and catalyst components in very small quantities. Analysis of the reaction mixtures
• Derivatization of natural products using biosynthetic and partial synthetic strategies. Biological testing of substances as antibiotics or antitumor compounds. Partly very small amounts and additional purification required.
• transition metal bioconjugates
• Efficient analysis of very small quantities with very complex substances
• Purification of peptide derivatives
• Analysis of the identity and purity of chiral molecules